Native bacteria and cyanobacteria can influence seedling emergence and growth of native plants used in dryland restoration

1. Seed-based ecosystem restoration has huge potential to restore degraded drylands. However, fewer than 10% of directly sown seeds transition to established seedlings. One of the potential factors restricting plant establishment in degraded soils is the low abundance and diversity of native soil micro-organisms. In this study, we investigated whether returning indigenous bacteria and cyanobacteria consortia to degraded dryland soils improved seedling emergence, survival and growth of native plants. 2. We inoculated ‘culturable whole soil’ native heterotrophic bacteria and biocrust cyanobacteria individually and as a mixed inoculant into extruded pellets containing Acacia inaequilatera (Fabaceae) and Triodia epactia (Poaceae) seeds. The pellets were planted in an active minefield for 28 weeks and seedling emergence and total biomass of plants were determined. 3. Cyanobacteria and bacteria inoculants increased the emergence of A. inaequilatera by 55% and 48%, respectively. Seedling emergence in T. epactia was increased by 20% by cyanobacteria but was not increased by bacteria. The only effect of inoculation on seedling survival or mass per surviving seedling in either species was an 11% reduction of the growth of T. epactia seedlings that were inoculated with cyanobacteria. 4. Synthesis and applications: Our results suggest that the benefit of micro-organisms on plant establishment is both species specific and life stage specific, with particularly strong benefits in the early stages of recruitment. Our experiment was conducted under shade and with additional water, so a worthwhile future direction would be to quantify the effect of inoculation under unmodified field conditions. It would also be worthwhile monitoring the outcomes for longer than 28 weeks. Since seedling emergence is one of the critical challenges in dryland restoration, our study provides direct evidence in the use of native micro-organisms to potentially improve seedling emergence in seed-based dryland restoration.

Australian Research Council Discovery Early Career Research Award - DE180100570

Hermon Slade Foundation de Australia - HSF 1808

Department of Industry, Innovation and Science, Australian Government's Global Innovation Linkages - GIL 53873

Ministerio de Ciencia e Innovación de España, beca Ramón y Cajal - RYC2020-029255-I