Trichoderma asperellum, Bacillus subtilis un to konsorcija ietekme uz augsnes mikroorganismiem | Influence of Trichoderma asperellum and Bacillus subtilis, and their consortium on soil microorganisms

Pētījuma mērķis bija noskaidrot, vai Trichoderma asperellum, Bacillus subtilis un to konsorcijs ietekmē augsnes mikrobiotu. Pētījumā eksperimentālie augsnes paraugi tika apstrādāti ar B. subtilis, T.asperellum un B. cinerea mikroorganismiem un to konsorcijiem. Izmantojot mikrobioloģiskās metodes, tika noteikts baktēriju un sēņu daudzums dažādi apstrādātās augsnēs. Ar Biolog EcoPlates metodi, noteikta mikroorganismu metaboliskā aktivitāte augsnē. Trichoderma ģints, Firmicutes, Gammaproteobacteria un Acidobacteria DNS koncentrācija augsnes paraugos tika noteikta ar molekulārajām metodēm. Tika veikta korelācijas analīze starp mikrobioloģisko un molekulāro un tikai starp dažādiem molekulāro rezultātu datiem. Augsnes apstrāde ar dažādiem mikroorganismiem un to konsorcijiem kā palielināja, tā arī samazināja noteiktu taksonu kolonijas veidojošo vienību skaitu un amplificēto DNS fragmentu daudzumu augsnē. Mikroorganismi un to konsorciji palielināja ogļhidrātu un komplekso oglekļa savienojumu izmantošanu, bet samazināja organisko fosfora savienojumu izmantošanu augsnē. Palielinoties KVV/g, palielinājās arī amplificēto DNS fragmentu koncentrācija noteiktos paraugos pēc noteikta dienu skaita.

The goal of the study was to determine if T.asperellum, B.subtilis and their consortia affect the microorganisms of the soil. During the study, experimental soil samples were prepared and treated with B.subtilis, T.asperellum, B.cinerea and their consortia. The amount of bacteria and fungi in soil samples that underwent different treatments was determined by microbiological methods. Using the Biolog EcoPlates method, the ability of the metabolic activity of microorganisms in soil was determined. The concentration of Trichoderma genus, Firmicutes, Gammaproteobacteria and Acidobacteria DNA in soil samples was determined by molecular methods. A correlation analysis was performed between microbiological and molecular data and only between molecular results data. Soil treatment with certain microorganisms and their consortia increased or decreased the amount of certain taxon-forming units and amplified DNA fragments in the soil. Various microorganisms and their consortia increase the utilisation of carbohydrates and complex carbon compounds but reduce the use of organic phosphorus compounds in the soil. Increasing CFU/g will also increase the concentration of amplified DNA fragments in certain samples after a certain number of days.