Root and crown rot of strawberry caused by Pythium helicoides and its distribution in strawberry production areas of Japan
Ishiguro, Y. (Gifu University, Gifu (Japan). River Basin Research Center) | Otsubo, K. | Watanabe, H. | Suzuki, M. | Nakayama, K. | Fukuda, T. | Fujinaga, M. | Suga, H. | Kageyama, K.
Pythium species were isolated from seedlings of strawberry with root and crown rot. The isolates were identified as P. helicoides on the basis of morphological characteristics and sequences of the ribosomal DNA internal transcribed spacer regions. In pathogenicity tests, the isolates caused root and crown rot similar to the original disease symptoms. Multiplex PCR was used to survey pathogen occurrence in strawberry production areas of Japan. Pythium helicoides was detected in 11 of 82 fields. The pathogen is distributed over six prefectures.
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