A globotetraosylceramide (Gb sub(4)) receptor-based ELISA for quantitative detection of Shiga toxin 2e
2015
Togashi, K. (Yamagata Integrated Agricultural Research Center Swine Experiment Station, Yamagata (Japan)) | Sasaki, S. | Sato, W.
Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosylceramide (Gb sub(4)) receptor by ELISA (Gb sub(4)-ELISA). No cross-reactivity was found with the other Shiga toxins Stx1 and Stx2, indicating high specificity. When the recombinant Stx2e B subunit (Stx2eB) was used, the absorbance measured by Gb sub(4)-ELISA increased linearly with Stx2eB concentration in the range of 20-2,500 ng/ml. The Gb sub(4)-ELISA method can be easily performed, suggesting that it would be a useful diagnostic tool for porcine edema disease.
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