Effect of alpha-lactoalbumin on the frequent urination induced by cyclophosphamide in rats
2017
Uchida, M. (Meiji Co., Ltd., Odawara City, Kanagawa (Japan). R and D Div., Food Science Research Labs.) | Kobayashi, O.
Frequent urination is a serious problem and remarkably decrease the Quality of Life. However, frequent urination has been also reported as an adverse effect in anti-cancer drugs therapy using cyclophosphamide and ifosfamide. Then, the present study aimed to investigate the effect of alpha-lactoalbumin on the frequent urination induced by cyclophosphamide in Sprague-Dawley strain female rats. Cyclophosphamide dissolved in saline was administered intraperitoneally at doses of 50 and 150 mg/kg. Distilled water for injection was administered orally in a volume of 20 ml/kg after 2 days of cyclophosphamide-treatment. Thereafter, urination number of times and urine volume were measured every 15 min till 120 min. At a dosage of 150 mg/kg, significant increase of urination number of times and significant decrease of the average urination volume were observed, but not at 50 mg/kg. Then, the effect of alpha-lactoalbumin was evaluated by the administration of 150 mg/kg of cyclophosphamide. alpha-Lactoalbumin and dexamathason were administered orally at a dosage of 1000 and 5 mg/kg, respectively, before and after cyclophosphamide administration. Urination test was performed 2 days after cyclophosphamide. alpha-Lactoalbumin and dexamathason markedly decreased the urination number of times, and at 120 min alpha-lactoalbumin showed a significant decrease (p<0.05). Moreover, alpha-lactoalbumin significantly attenuated the significant decrease of the average urination volume induced by cyclophosphamide. Dexamathason also showed almost the same efficacy as alpha-lactoalbumin. These results show that alpha-lactoalbumin is effective on the frequent urination induced by cyclophosphamide.
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