Supplementary effect of hydrogen peroxide as a pre-disinfectant for sterilizing rhizome bud explants of Zantedeschia aethiopica L. with chlorine dioxide
2017
Chen, L.R. (Chinese Culture University, Taipei (Taiwan). Department of Horticulture and Biotechnology) | Hsiung, T.C. | Lin, K.H. | Huang, T.B. | Huang, M.Y. | Wakana, A.
Zantedeschia spp. (calla lily) are ornamental tuberous/rhizomatous plants of economic importance worldwide. In vitro micropropagation techniques have been widely used to mass-produce virus-free calla lilies. However, effective removal of contaminants is a major problem for the in vitro establishment of the calla lily propagation. In this study, we applied hydrogen peroxide (H2O2) as a pre-disinfectant for sterilizing rhizome bud explants of Z. aethiopica followed by sterilization with sodium hypochlorite (NaOCl) or chlorine dioxide (ClO2) to eliminate in vitro contamination. Results showed that application of 5% (w/v) H2O2 for 5 minutes followed by 1% (w/v) NaOCl or 60-180 mg./L ClO2 for 15 minutes significantly reduced the contamination rate to < 38% while maintaining the vigor of explant tissues. Surviving explants sterilized with H2O2 followed by ClO2 exhibited a higher rate (>75%) of those showing shoot development than those sterilized with NaOCl (54%). The two-step sterilization method with H2O2 and ClO2 synergistically optimized the disinfection efficiency and explants viability.
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