New tri-parental interspecific Torenia hybrids via ovule culture
2020
Laojunta, T. | Narumi-Kawasaki, T. | Fukai, S.
Tri-parental cross is an effective way to increase variations in flower color and morphological characteristics. Recombination of three genomes would produce new phenotypes having high ornamental value. The fertile pollen of (T. bicolor × T. baillonii) was mounted on the stigma of four species, T. concolor, T. fournieri, T. hirsutissima and T. siamensis. Only the cross combination of T. fournieri × (T. bicolor × T. baillonii) produced five progenies. Then three hybrids (T. fournieri × T. baillonii), (T. fournieri × T. bicolor) and (T. bicolor × T. baillonii) were used as female parents then cross with other Torenia species. Three progenies were obtained from (T. fournieri × T. baillonii) × T. siamensis and two progenies were obtained from (T. fournieri × T. baillonii) × T. concolor. The hybridity of progenies was confirmed by PCR-RFLP, and all tested plants were true hybrids. Tri-parental hybrids showed intermediate chromosome number between parents and all tri-parental hybrids were male sterile. Petal color of the hybrids was mostly intermediate of parents with narrow variation.
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