Advanced Genetic Studies on Powdery Mildew Resistance in TGR-1551
2022
López-Martín, María | Pérez-de-Castro, Ana | Picó, Belén | Gómez-Guillamón, María Luisa | Ministerio de Ciencia e Innovación (España) | Generalitat Valenciana | Ministerio de Ciencia, Innovación y Universidades (España) | Agencia Estatal de Investigación (España) | 0000-0001-7528-8844 | 0000-0002-4949-3323 | Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
Cucurbits powdery mildew (CPM) is one of the main limiting factors of melon cultivation worldwide. Resistance to races 1, 2, and 5 has been reported in the African accession TGR-1551, whose resistance is controlled by a dominant-recessive epistasis. The dominant and recessive quantitative trail loci (QTL) have previously been located in chromosomes 5 and 12, respectively. We used several densely genotyped BC3 families derived from the cross between TGR-1551 and the susceptible cultivar 'Bola de Oro' to finely map these resistance regions. The further phenotyping and genotyping of the selected BC5, BC5S1, BC5S2, BC4S1, BC4xPS, and (BC4xPS) S1 offspring allowed for the narrowing of the candidate intervals to a 250 and 381 kb region in chromosomes 5 and 12, respectively. Moreover, the temperature effect over the resistance provided by the dominant gene has been confirmed. High resolution melting markers (HRM) were tightly linked to both resistance regions and will be useful in marker-assisted selection programs.
Показать больше [+] Меньше [-]This research was funded by the Spanish Ministerio de Ciencia e Innovación (MCIN/AEI/10.13039/501100011033), grant number PID2020-116055RB (C21 and C22), and by the Conselleria d’Educació, Investigació, Cultura i Esports de la Generalitat Valenciana, grant number PROMETEO/2021/072 (to promote excellence groups, cofinanced with FEDER funds). M.L. is a recipient of a predoctoral fellowship (PRE2018-083466) of the Spanish Ministerio de Ciencia, Innovación y Universidades co-financed with FSE funds.
Показать больше [+] Меньше [-]Peer reviewed
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