Biostimulation with the ram effect increases the embryo quality in superovulated ewes.

SCHMIDT, A. P. S.; ARRAIS, A. M.; BRANDÃO, F. Z.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. DA; UNGERFELD, R.; ANA PAULA PEREIRA SCHMIDT, UNIVERSIDADE FEDERAL FLUMINENSE; ALINE MATOS ARRAIS, UNIVERSIDADE FEDERAL FLUMINENSE; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA EMILIA FRANCO OLIVEIRA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA GONÇALVES SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; RODOLFO UNGERFELD, UNIVERSIDAD DE LA REPÚBLICA.

Biostimulation with the ram effect increases the embryo quality in superovulated ewes.

2024

SCHMIDT, A. P. S. | ARRAIS, A. M. | BRANDÃO, F. Z. | BATISTA, R. I. T. P. | OLIVEIRA, M. E. F. | SOUZA-FABJAN, J. M. G. | FONSECA, J. F. DA | UNGERFELD, R. | ANA PAULA PEREIRA SCHMIDT, UNIVERSIDADE FEDERAL FLUMINENSE; ALINE MATOS ARRAIS, UNIVERSIDADE FEDERAL FLUMINENSE; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA EMILIA FRANCO OLIVEIRA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA GONÇALVES SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; RODOLFO UNGERFELD, UNIVERSIDAD DE LA REPÚBLICA.

The pulsatile release of luteinizing hormone (LH) induced by the male effect may play a beneficial role in embryonic viability during superovulation. The objective of the present study was to determine if biostimulation with males for 72 h enhances the ewes’ superovulatory response, including the embryo quality. The data were analyzed using mixed models with Bioestat statistical software, including the treatments, time, and their interactions as the main effects. Data are presented as LS mean ± SEM. Differences were considered significant when P ≤ 0.05. No difference was observed in the means of ovarian cysts (0.2±0.1 vs. 0.9±0.5), CLs (9±1 vs. 8±1), recovered structures (6±1 vs. 5±1), viable structures (4±1 vs. 3±1 in CG and REG treatments. However, bioestimulation increased the abundance of transcripts associated with energy metabolism (SIRT2) and cell adhesion (CDH1) and reduced the expression of those related to stress and apoptosis (PRDX.1 and BCL2). In conclusion, biostimulation promoted healthier embryo development according to gene expression, impacting positively therefore the quality of the embryos recovered.

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Edição dos resumos da 37ª Annual Meeting of the Brazilian Embryo Technology Society, 2024, Atibaia, SP.

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