Fine Mapping of <i>Bush</i> Gene and Development of Molecular Marker for Bush Type in Pumpkin (<i>Cucurbita maxima</i> Duch.)
Chun Li | Zhen He | Yifan Li | Yanhong Lan | Fangyi Gong | Peng Cai | Nan Yang | Yan Liu | Feng Xia | Genyun Liang | Xiaojun Liu
The bush trait is an important agronomic trait with great value for pumpkin crops. In this study, a bush-type line, CS82, identified in pumpkin (<i>Cucurbita maxima</i>) showed no obvious main vine, and all petioles clustered at the extremely shortened stem with limited internodes. The microscopy analysis revealed that the bush-type phenotype may be due to the degeneration of the shoot apex. Genetic analysis showed that the bush-type phenotype is controlled by a single dominant nuclear gene. Exogenous gibberellin treatment could not recover the bush-type phenotype to the wild type, indicating that the bush-type phenotype is not due to the mutation of gibberellin biosynthesis genes. The BSA-seq analysis preliminarily mapped the <i>Bush</i> gene to Chr.15 of the pumpkin genome. Further fine mapping limited the <i>Bush</i> gene to a physical distance of 95.65 kb with 19 genes. Based on the gene function and the 63 bp deletion, <i>CmaCh15G011490</i>, encoding an axial regulator YABBY 5-like protein, was selected as the candidate gene for the <i>Bush</i> gene. A quick and efficient method was developed for bush-type phenotype identification, which is useful for bush-type variety breeding in pumpkin.
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