Influence of plant regulators on the micropropagation of Echinacea purpurea ‘Raspberry Truffle’
Nabilah A. Samsurizal | Karolina Nowakowska | Andrzej Pacholczak
A micropropagation protocol was conducted for Echinacea purpurea ‘Raspberry Truffle’ to determine the influence of medium amendment with a plant growth regulator (PGR). The efficacy of meta-Topolin (mT) and benzyladenine (BA) was evaluated during the proliferation stages. Additionally, indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) were assessed during the rooting stages. Multiple shoots were initiated and proliferated on a Murashige and Skoog (MS) medium and supplemented with 1 mL·L–1 of a Plant Preservative Mixture (PPM) and selected plant growth regulators with concentrations of 0.5, 1 and 2 mg·L–1 for shoot proliferation; and 0.05, 0.1, 0.5 and 1 mg·L–1, for rooting performance. The combination of mT and BA in the medium significantly enhanced shoot regeneration and elongation. Both, mT and BA resulted in 100% shoot regeneration. mT at a concentration of 0.5 mg·L–1 in the MS medium induced the maximum number of shoots, followed by 0.5 mg·L–1 BA. The supplementation of 0.05 mg·L–1 and 1 mg·L–1 IBA, and 0.1 NAA mg·L–1 resulted in a 100% root percentage with the highest number of roots found in the media amended with 1 mg·L–1 IBA and 0.1 mg·L–1 NAA.
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