Optimizing Exome Captures in Species with Large Genomes Using Species-specific Repetitive DNA Blocker
2025
Kesälahti, Robert | Kumpula, Timo A. | Cervantes, Sandra | Kujala, Sonja T. | Mattila, Tiina M. | Tyrmi, Jaakko S. | Niskanen, Alina K. | Rastas, Pasi | Savolainen, Outi | Pyhäjärvi, Tanja | https://orcid.org/0000-0003-0949-6156 | 4100210410 | Luonnonvarakeskus
Large and highly repetitive genomes are common. However, research interests usually lie within the non-repetitive parts of the genome, as they are more likely functional, and can be used to answer questions related to adaptation, selection and evolutionary history. Exome capture is a cost-effective method for providing sequencing data from protein-coding parts of the genes. C0t-1 DNA blockers consist of repetitive DNA and are used in exome captures to prevent the hybridisation of repetitive DNA sequences to capture baits or bait-bound genomic DNA. Universal blockers target repetitive regions shared by many species, while species-specific c0t-1 DNA is prepared from the DNA of the studied species, thus perfectly matching the repetitive DNA contents of the species. So far, the use of species-specific c0t-1 DNA has been limited to a few model species. Here, we evaluated the performance of blocker treatments in exome captures of Pinus sylvestris, a widely distributed conifer species with a large (> 20 Gbp) and highly repetitive genome. We compared treatment with a commercial universal blocker to treatments with species-specific c0t-1 (30,000 and 60,000 ng). Species-specific c0t-1 captured more unique exons than the initial set of targets leading to increased SNP discovery and reduced sequencing of tandem repeats compared to the universal blocker. Based on our results, we recommend optimising exome captures using at least 60,000 ng of species-specific c0t-1 DNA. It is relatively easy and fast to prepare and can also be used with existing bait set designs.
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Эту запись предоставил Natural Resources Institute Finland