Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress
Křcková, Zuzana | Brouzdová, Jitka | Daňek, Michal | Kocourková, Daniela | Rainteau, Dominique | Ruelland, Eric | Valentová, Olga | Pejchar, Přemysl | Martinec, Jan | Czech Academy of Sciences [Prague] (CAS) | University of Chemistry and Technology Prague (UCT Prague) | Microorganismes, Molécules Bioactives et Physiopathologie Intestinale (LBM-E4) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Université Pierre et Marie Curie - Paris 6 (UPMC) | Institut d'écologie et des sciences de l'environnement de Paris (iEES) ; Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)
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Показать больше [+] Меньше [-]Английский. The Arabidopsis non-specific phospholipase C 1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
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