Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities
2021
Darsonval, Maud | Grégoire, Marina | Deschamps, Julien | Briandet, Romain | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | This project was funded by the French National Agency for Research (program "PathoFood ANR-17-CE21-0002") and by AgroParisTech (program "SBAO19-2"). Microscopic images were performed at the INRA MIMA2 imaging center. We thank Kevin Bascaran for technical assistance. | Springer | ANR-17-CE21-0002,PathoFood,Décrypter l'hétérogénéité cellulaire et la distribution spatiale de pathogènes dans les matrices alimentaires en interaction avec les communautés microbiennes(2017)
International audience
Показать больше [+] Меньше [-]Английский. The behavior of Listeria monocytogenes communities in the food chain is closely associated with their spatial organization. Whether as biofilms on industrial surfaces or as microcolonies in food matrices, the resulting physiological diversification combined with the presence of extracellular polymeric substances (EPS) triggers emergent community functions involved in the pathogen survival and persistence (e.g., tolerance to dehydration, biocides, or preservatives). In this contribution, we present a noninvasive confocal laser microscopy (CLM) protocol allowing exploration of the spatial organization of L. monocytogenes communities on various inert or nutritive materials relevant for the food industry.
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