Effects of Lactobacillales supplementation in rat model of short bowel syndrome (SBS)
2024
Garrigues, Alice | Bourgin, Mélanie | Pourtier, Nicolas | Savary-Auzeloux, Isabelle | Ribeiro, Lara | Roy, Maryline | Dumay, Anne | Kapel, Nathalie | Bado, Andre | Le Gall, Maude | Thomas, Muriel | Le Beyec, Johanne | Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité) | Unité de Nutrition Humaine (UNH) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA) | Université Clermont Auvergne (UCA) | Service de Chirurgie Générale et Digestive [Hôpital Bichat - Claude Bernard, Paris] ; AP-HP - Hôpital Bichat - Claude Bernard [Paris] ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP) | Physiopathologie et pharmacotoxicologie placentaire humaine : Microbiote pré & post natal (3PHM - UMR-S 1139) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité) | CHU Saint-Antoine [AP-HP] ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) | Sorbonne Université - Faculté de Santé [CHU Pitié Salpétrière] ; CHU Pitié-Salpêtrière [AP-HP] ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | CHU Pitié-Salpêtrière [AP-HP] ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) | Université Pierre et Marie Curie - Paris 6 (UPMC) | European Society for Clinical Nutrition and Metabolism
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Показать больше [+] Меньше [-]Английский. Rationale: Microbiota composition of patients with Short Bowel Syndrome (SBS) is characterized by a high prevalence ofLactobacillus. Transferring this microbiota to germ-free rats induced certain adaptations observed in SBS patients, suchas colonic hyperplasia. In this study, 2 bacterial strains isolated from this SBS-microbiota were used to test theirindividual effects on the setting of adaptations in a rat model of SBS.Methods: Wistar rats underwent 80% resection of the small intestine, ileocecal valve and right colon or intestinaltransection and received a daily supplementation with either strain A or B for 28 days. Body weight, food intake andfaecal appearance were measured daily. Faeces and blood were collected for microbiome and gastrointestinal hormoneanalyses respectively. Digestive transit time was evaluated using carmine red. Jejunum and colon were sampled formorphometric analyses. Results are expressed as mean ±SEM and statistical analyses used non parametric tests (2-wayand 1-way ANOVA, Kruskal-Wallis test).Results: Supplementation with the 2 strains had no impact on microbiota diversity and composition compared withuntreated SBS rats. All SBS rats lost weight the 1rst week and then regained some, with no difference between SBSgroups. SBS (p=0.0201) and SBS+A (p=0.0207) rats exhibited a significant increase of food intake compared to SHAMrats whereas SBS+B rats did not (p=0.7249). At D28, SBS treated rats had less diarrhoea and a slowed transit timecompared to untreated SBS rats. The thickness of the jejunal muscular layer was significantly increased in the SBStreated groups (p<0,001).Conclusion: This study indicates a beneficial effect of these probiotics since supplementation with strain A or B led todiarrhoea improvement, suggesting a reduction in water loss. Furthermore, the treatment helped slowing down the transittime, allowing longer contact between food and the absorption surface.
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