Abundance, cell volume, biomass, viability and leucine incorporation rates of prokaryotes in the tropical and subtropical Atlantic

Samples were collected in the tropical and subtropical Atlantic during the MAFIA cruise (April 2015) on board the BIO Hespérides. Seawater samples were collected at 13 stations (from the Brazilian coast to the Canary Islands), from the surface down to 3500 m, using a General Oceanics oceanographic rosette equipped with 24 l PVC Niskin bottles. Abundance and cell characteristics (high nucleic acid content fraction, cell volume, viability) were based on measurements performed with a FACSCalibur (Becton-Dickinson) flow cytometer. Leucine incorporation rates were estimated with tritiated leucine (Kirchman et al. 1985) using centrifugation and filtration methods (Smith and Azam 1992). The aim of this dataset was to estimate the influence of surface productivity on the standing stock, characteristics and activity (as leucine incorporation) of prokaryotes across the water column

This dataset contains the results of the characterisation of the prokaryotic community by flow cytometry and tritiated leucine incorporation from the MAFIA cruise (Migrants and Active Flux In the Atlantic ocean)

Horizon 2020 (H2020), grant/award no. 817806: Sustainable management of mesopelagic resources; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2012-39587-C04: Migrants and Active Flux In the Atlantic Ocean; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2015-69392-C3: Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2017-83362-R: INTERES: Papel de las interacciones fitoplancton-bacterias en la respuesta del plancton microbiano a la entrada de nutrientes alóctonos; Ministerio de Ciencia e Innovación (MICINN), grant/award no. PID2019-109084RB-C21: Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity

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