SAUR63 fusion protein lipid binding
2022
Nagpal, Punita | Reeves, Paul H. | Wong, Jeh Haur | Armengot, Laia | Chae, Keun | Rieveschl, Nathaniel B. | Trinidad, Brendan | Davidsdottir, Vala | Jain, Prateek | Gray, William M. | Jaillais, Yvon | Reed, Jason W.
1 figure.
Показать больше [+] Меньше [-]A) Western blot showing presence of fusion proteins in extracts used in lipid blot experiments in Fig 5A. Arrows indicate locations of full-length SAUR63:YFP:HA and truncated SAUR6326-142:YFP:HA fusion proteins (upper arrow) and SAUR631-25:YFP:HA N-terminal domain fusion protein (lower arrow). B) Longer exposures of two lipid blots from Fig 5A. C) Mock experiment in which extracts were incubated for 70 minutes in protein extraction buffer at the indicated temperatures, and then run on a gel for western blots. For both SAUR63:YFP:HA and SAUR6326-142:YFP:HA, similar amounts of protein are present after incubation at -20 C or after incubation at 22 C, as during the lipid blot binding experiment.
Показать больше [+] Меньше [-]Peer reviewed
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