A PCR-microarray method for the screening of genetically modified organisms
Hamels, Sandrine | Glouden, Thomas | Gillard, Karine | Mazzara, Marco | Debode, Frédéric | Foti, Nicoletta | Sneyers, Myriam | Esteve, Teresa | Pla, María | Berben, Gilbert | Moens, William | Bertheau, Yves | Audéon, Colette | Eede, Guy van den | Remacle, José | European Commission
A new method to screen and to identify genetically modified organisms (GMO) is presented in this paper. It is based on the detection of multiple genetic elements common to GMO by their amplification via PCR followed by direct hybridisation of the amplicons on microarray. The pattern of the elements is then compared to a database of the composition of EU-approved GMO and an identification of the GMO is then proposed. The limit of detection of the method was ≤0.1% GMO content (w/w) expressed as the amount of target DNA present in the template for single unprocessed material. The DNA targets were detected both in reference materials and in mixtures with the same detection limit. The specificity for the detection of the different elements was found to be very good with no cross-reaction even in samples with two GMO present at different concentrations. The paper presents examples of GMO identification and discusses the potential and limitation of such approaches and how they can facilitate the work of private and enforcement detection laboratories.
Показать больше [+] Меньше [-]This study was supported by the European Commission through the FP5 program “GMOchips” (contract G6RD-CT2000-00419 2000-2003), the Belgian SSCT program and the Integrated Project “Co-Extra”, contract no. 007158 2005-2009, under the 6th Framework Programme, priority 5, food quality and safety.
Показать больше [+] Меньше [-]Peer reviewed
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