Timing of defoliation affects anthocyanin and sugar decoupling in Grenache variety growing in warm seasons

Warming trends over the winegrowing regions lead to an advance of grapevine phenology, decreased yield and increased sugar content with a lower polyphenol content. We hypothesized that different leaf removal timings may counteract these effects. A two-year experiment was conducted in La Rioja (Spain) with Vitis vinifera L. cv. Grenache trained in an open-vase system. Trial consisted in a complete block design with two leaf removal treatments differing in the moment of manipulation: i) severe leaf removal treatment conducted after fruit set (ELR); and ii) severe leaf removal at veraison (LLR) compared to an untreated control (Control). Both leaf removal treatments tended to decrease sugar content with no effect on yield, these effects being highly affected by the year. Defoliation accounted for a decreased flavanol and stilbene contents in berries at harvest. An ELR increased anthocyanin and phenolic acid contents at harvest, while warming during 2022 accounted for decreased contents of all the monitored groups of flavonols. ELR was only effective for delaying ripening by means of impairing the sugar:anthocyanin decoupling during the 2021 growing season which was related to lower % of kaempferol. Altogether, results suggested that defoliation should still be applied under currently warming trends in some viticulture regions.

This work was performed with the financial support of AGL2017–87373-C3–3-R. A. Villa-Llop is beneficiary of an Industrial pre-doctoral contract of the Government of Navarra (Ref. 283E/2020). N. Torres is beneficiary of a Ramón y Cajal Grant RYC2021–034586-I funded by MCIN/AEI/ 10.13039/501100011033 and by “European Union NextGenerationEU/PRTR”. Open access funding provided by Universidad Pública de Navarra.

Peer reviewed