Application of pressurized liquid extractions to obtain bioactive compounds from Tuber aestivum and Terfezia claveryi

This article belongs to the Special Issue Extraction, Characterization and Biological Activity of Food Bioactive Compounds.

A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investigated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, β-glucans, chitin, proteins, phenolic compounds and sterols). Principal component analysis indicated water solvent and high temperatures as more adequate parameters to extract polysaccharide-rich fractions (up to 68% of content), whereas ethanol was more suitable to extract fungal sterols (up to 12.5% of content). The fractions obtained at optimal conditions (16.7 MPa, 180 °C, 30 min) were able to protect Caco2 cells from free radical exposure, acting as antioxidants, and were able to reduce secretion of pro-inflammatory cytokines in vitro: IL-6 (50%), and TNFα (80% only T. claveryi ethanol extract), as well as reduce high inhibitory activity (T. aestivum IC50: 9.44 mG/mL).

This research was funded by fellowship Ibercaja-CAI Estancias de Investigacióngrant number [CA 120] And The APC was funded by Fondo Europeo Agrícola de Desarrollo Rural, Programa de Desarrollo Rural de la Región de Murcia (2014–2020) and Diputación Provincial de Zaragoza (DPZ) (Spain).

Peer reviewed