Effect of germination, under different environmental conditions, on saponins, phytic acid and tannins in lentils (Lens culinaris)
Ayet, G. | Burbano, C. | Cuadrado Hoyos, María Carmen | Pedrosa, M. M. | Robredo, L. M. | Muzquiz, M. | De la Cuadra, C. | Castaño, A. | Osagie, Anthony
The effect of germination conditions on some antinutrients of Lens culinaris var Magda 20 seeds were studied. The seeds were germinated at 20°C under variable conditions of time, water and light. Quantitative analyses of the soyasapogenols, inositol phosphates and tannins were carried out by capillary gas chromatography, high-performance liquid chromatography and spectrophotometric techniques respectively. Germinated seeds at day 6 contained higher levels soyasapogenol B than the controls, whereas in general the tannin content was reduced. Total phytic acid amounts did not decrease after 3 days of germination but was greatly reduced after 6 days. This work shows that the optimal conditions to reduce some antinutritional factors (tannins and phytic acid) in lentils were 6 days of seed germination in dark and with alternate watering. Therefore, germination conditions offer a good opportunity to improve the nutritional quality of lentils.
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