Essay of different extraction procedures in capelin fish meal for biogenic amine determination by HPLC
Ruiz-Capillas, Claudia | Triki, Mehdi | Heras, Cristina de las | Tejada Yábar, Margarita | Pálmadóttir, Heiða | Porvaldsdóttir, Rósa | Jiménez Colmenero, Francisco | Herrero, Ana M.
This study assesses the effects of different extraction procedures of biogenic amines in capelin fish meal with different concentrations of trichloroacetic acid (TCA) for their determination by high performance liquid chromatography (HPLC). No significant differences were noted between the use of TCA at 7.5 and 10% for the extraction of biogenic amines in E1 and E3 extraction, except for tyramine. The extraction using 7.5% TCA in one step and centrifugation (E1) was a simple and rapid method. This extraction seems to be the most suitable for the extraction of amines in these kinds of samples, compared to the other methods tested. The biogenic amine that presented the highest levels in the fish meal studied was cadaverine (208-226 mg/kg), followed by putrescine and histamine (164-173 and 44-46 mg/kg, respectively). Biogenic amine levels were low when compared to the toxic levels observed in these kinds of products.
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