The relationship of antioxidant vitamins intake and cytokine profile in In vitro response

Resumen del trabajo presentado al International Symposium on Immunonutrition (10th Anniversary), celebrado en Madrid (España) del 17 al 19 de julio de 2017.

[Background and Objective]: Vitamins A, C and E deficits can lead to alterations in the immune system and cytokine dysregulation. This study was aimed to evaluate the relationship of daily intake of these vitamins and the in vitro effect of tomato extract (TE) addition on cytokine production by peripheral blood mononuclear cells (PBMCs).

[Methods]: Human PBMCs (n = 7, women’s independent samples) were isolated and cultured with PHA and LPS for 48 hs to stimulate cytokine production. Four groups were considered: PBMCs + PHA/LPS + 1, 10 or 20 μM of α-tocopherol from TE (PBMC-1, PBMC-10 and PBMC-20 groups, respectively) and PBMCs + PHA/LPS used as the control group (C). IFN-γ, TNF-α, IL-1β, and IL-10 were measured in culture supernatants by flowcytometry (CBA, EMDMillipore). The daily vitamin intakes were analyzed from a Food frequency questionnaire. Statistics were performed using SPSS 23. Kolmogorov-Smirnov was applied to assess normality and Pearson correlations were established.

[Results]: Vitamin A and E intakes were positively correlated with IL-10 in PBMC-1, PBMC-10, PBMC-20 and C group (p = 0.029; p = 0.025; p = 0.013; p = 0.028 and p = 0.008; p = 0.005; p = 0.003; p = 0.006), respectively. TNF-α showed a positive correlation with vitamin C (p = 0.037; p = 0.013) in PBMC-10 and PBMC-20 groups. TNF-α/IL-10 ratio showed a negative correlation with vitamin A and E intake levels (p = 0.033; p = 0.011 and p = 0.045; p = 0.022) in PBMC-1 and PBMC-20 groups, respectively.

[Conclusions]: Antioxidant vitamin intakes affect the response of PBMCs to mitogens, being involved in the regulation of pro and anti-inflammatory cytokines.

This work was supported by the Intramural Proyect, CSIC (grant number 201270E121) and the Postgraduate and Advanced Training Programs, INTA (MFG, grant number 1177/14).

Peer reviewed