Propionibacterium olivae sp. nov. and Propionibacterium damnosum sp. nov., Isolated from spoiled packaged Spanish-style green olives

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Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44% with Propionibacterium acidipropionici NCFB 563 and 98.33 and 98.11% with Propionibacterium microaerophilum M5T, respectively, and a similarity of 99.41% between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from P. acidipropionici NCFB 563 and P. microaerophilum M5T. Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus Propionibacterium. Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus Propionibacterium, for which the names Propionibacterium olivae sp. nov. (type strain, IGBL1T5CECT 8061T5DSM 25436T) and Propionibacterium damnosum sp. nov. (type strain, IGBL13T5CECT8062T5DSM 25450T) are proposed.

This research was funded by the Spanish Ministry of Science and Innovation (MICINN) and the Spanish Ministry of Economy and Competitiveness (MINECO) through projects AGL2009-07861 and AGL2012-33400, respectively, and by the Junta de Andalucía Excellence Projects AGR-04621 and AGR-07345, all of them including FEDER funds. A. M. B. and H. L. P. were the recipients of grants awarded by the Spanish National Research Council (CSIC) through the JAE Doc and JAE predoctoral programmes, respectively.

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