Biocontrol of plant pathogens by Lysobacter enzymogenes isolate LEC: efficacy of shake flask broth, fermentation broth and dried preparations
2025 | 2024
Drenker, Christian | El Mazouar, Doris | Seib, Tanja | Weißhaupt, Sonja | Stephan, Dietrich | Kunz, Stefan | Reineke, Annette | Rondot, Yvonne | Linkies, Ada | Koch, Eckhard
The aim of our study was to upscale the production of Lysobacter enzymogenes strain LEC from shake flasks to a laboratory bioreactor. The obtained fermentation broth was to be downstream-processed by lyophilisation and fluidised bed-drying, followed by testing of the dried preparations against plant pathogens. Different batches of broth from shake flasks and from individual fermenter runs were characterised and compared. Number of viable cells, in vitro efficacy (ED90 value) against the apple scab pathogen Ventura inaequalis, as well as efficacy against Phytophthora infestans on potato plants were all reproducible and not impaired by upscaling the production volume from shake flask to the bioreactor. Fermentation broth was processed by lyophilisation and fluidised bed-drying. In bioassays, the activity against downy mildew (Pseudoperonospora cubensis) on cucumber were comparable for the unformulated fermentation broth and the fluidised bed-dried product, while the lyophilised product was significantly more effective. Strain LEC showed reproducible pathogen suppressive activity, independent from production scale and batch. Production upscaling and formulation by drying are possible without loss of efficacy.
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Библиографическая информация
Издатель Carfax Publ. Comp.
ISSN 0958-3157Эту запись предоставил Julius Kühn-Institut