Production and structural analysis of the polysaccharide secreted byTrametes (Coriolus) versicolor ATCC 200801
Rau, Udo | Kuenz, Anja | Wray, Victor | Nimtz, Manfred | Wrenger, Julika | Cicek, Hasan
Trametes versicolor ATCC 200801 secretes 4.1 g L-1 of exopolysaccharide (EPS) when synthetic minimal medium and low-shear bioreactor cultivation technique are used. Structural and compositional analyses by thin layer chromatography, gas chromatographymass spectrometry, electrospray ionization tandem mass spectrometry, and nuclear magnetic resonance spectroscopy yielded predominantly glucose and small amounts of galactose, mannose, arabinose, and xylose. The main EPS is composed of ß-1,3/ß-1,6-linked D-glucose molecules which is identical with Schizophyllan but does not possess a triple helical arrangement as secondary structure. Two molar mass fractions were detected by size exclusion chromatography yielding weight-average molecular weights of 4,100 and 2.6 kDa. Protein content varies between 23.6% (w/w). The exopolysaccharide is different in the nature of the glycosidic linkage, composition of monosaccharides, protein content, and weight-average molecular weight compared to the well-known polysaccharopeptide (PSP) and polysaccharopeptide Krestin (PSK).
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Библиографическая информация
Издатель Springer
ISSN 0175-7598 | 0171-1741 | 1432-0614Эту запись предоставил Thünen-Institut