Optimizing In Vitro Propagation of <i>Haworthia truncata</i> Schönland Using Leaf, Root, and Inflorescence
Leila Soleimani | Hassan Salehi | Taras Pasternak
<i>Haworthia truncata</i>, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting <i>Haworthia</i> globally. Tissue culture offers an efficient method for mass-producing unique and beautiful species such as <i>H. truncata.</i> This study tested Murashige and Skoog (MS) basal media supplemented with various concentrations of IBA (0.05–1.5 mg/L), NAA (0.05–0.25 mg/L), and BA (0.25–1.5 mg/L) to promote shoot proliferation. MS medium without plant growth regulators (PGRs) was also tested as a control. Different explant types (leaf, root, and inflorescence) were analyzed for their potential in direct and indirect regeneration. Inflorescence explants showed the highest callus induction with 1.5 mg/L IBA, while optimal shoot proliferation occurred at 1 mg/L IBA. Callus induction was optimal for leaf explants with 0.05 mg/L NAA and 0.25 mg/L BA, and shoot proliferation was highest at 0.05 mg/L NAA and 1 mg/L BA. Root explants achieved maximum callus induction with 0.25 mg/L BA and 0.25 mg/L NAA, with the best shoot proliferation using 0.05 mg/L NAA and 1 mg/L BA. The highest rooting percentage of regenerated shoots was obtained on ½ MS medium with 1.5 mg/L IBA.
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