Endothelium-dependent vasodilator and antioxidant properties of a novel enzymatic extract of grape pomace from wine in dustrial waste
Rodriguez-Rodriguez, Rosalia | Justo, Maria Luisa | Claro Cala, Carmen María | Vila, Elisabet | Parrado Rubio, Juan | Herrera González, María Dolores | Álvarez de Sotomayor Paz, María | Universidad de Sevilla. Departamento de Farmacología, Pediatría y Radiología | Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular | Universidad de Sevilla. Departamento de Farmacología | Instituto de Biomedicina de Sevilla (IBIS) | Universidad de Sevilla. CTS-1074: Inmunonutrición e inmunometabolismo | Universidad de Sevilla. AGR-212 : Tecnologia y aplicacion de enzimas | Universidad de Sevilla. CTS-178: Farmacología cardiovascular | Ministerio de Ciencia e Innovación de España
The aim of the present study was to evaluate the vascular effects of an enzymatic extract of grape pomace (GP-EE) on isolated arteries, focusing our attention on endothelium-derived relaxation and on its antioxidant properties. Grape pomace derived from wine making was extracted by an enzymatic process and its composition of polyphenols was evaluated by HPLC and ESI-MS/MS, detecting kaempferol, catechin, quercetin and procyanidins B1 and B2, trace levels of resveratrol and tracing out gallocatechin and anthocyanidins. GP-EE induced endothelium- and NO-dependent vasodilatation of both rat aorta and small mesenteric artery (SMA) segments and reduced Phe-induced response in aortic rings. Both ORAC and DPPH assays confirmed antioxidant scavenging properties of GP-EE, which also prevented O(2)(·-) production (assessed by DHE fluorescence) and contraction elicited by ET-1. These results provide evidence that GP-EE possesses interesting antioxidant and protective vascular properties and highlight the potential interest of this extract as a functional food.
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