Marker-assisted breeding of Musa balbisiana genitors devoid of infectious endogenous Banana streak virus sequences
Umber, Marie | Pichaut, Jean-Philippe | Farinas, Benoît | Laboureau, Nathalie | Janzac, Bérenger | Plaisir, Kaissa | Pressat, Gersende | Baurens, Franc-Christophe | Chabannes, Matthieu | Duroy, Pierre-Olivier | Guiougou, Chantal | Delos, Jean-Marie | Jenny, Christophe | Caruana, Marie-Line | Salmon, Frédéric | Teycheney, Pierre-Yves | Agrosystèmes tropicaux (ASTRO) ; Institut National de la Recherche Agronomique (INRA) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Vilmorin | Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Institut de Biotechnologie ; Université de Lausanne = University of Lausanne (UNIL) | European Regional Development Fund (FEDER)
The authors wish to thank Frédéric Bakry and Léonidas Féréol for providing some doubled haploid lines and Guillaume Fort for technical help.This paper is dedicated to the memory of Jacky Ganry.
Показать больше [+] Меньше [-]Английский. Breeding new interspecific banana hybrid varieties relies on the use of Musa acuminata and M. balbisiana parents. Unfortunately, infectious alleles of endogenous Banana streak virus (eBSV) sequences are present in the genome of Musa balbisiana genitors. Upon activation by biotic and abiotic stresses, these infectious eBSVs lead to spontaneous infections by several species of Banana streak virus in interspecific hybrids harboring both Musa acuminata and M. balbisiana genomes. Here we provide evidence that seedy M. balbisiana diploids display diverse eBSV allelic combinations and that some eBSVs differ structurally from those previously reported. We also show that segregation of infectious and non-infectious eBSV alleles can be achieved in seedy M. balbisiana diploids through self-pollination or chromosome doubling of haploid lines. We report on the successful breeding of M. balbisiana diploid genitors devoid of all infectious eBSV alleles following self-pollination and on the potential of breeding additional M. balbisiana diploid genitors free of infectious eBSVs by crossing parents displaying complementary eBSV patterns. Our work paves the way to the safe use of M. balbisiana genitors for breeding banana interspecific hybrid varieties with no risk of activation of infectious eBSVs.
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