Uji Toksisitas Akut Infusa Umbi Bit (Beta vulgaris) pada Mencit (Mus musculus) Betina | Acute Toxicity Test of Beet Tuber Infusion (Beta vulgaris) on Female Mice (Mus musculus)

Umbi bit (Beta vulgaris) merupakan tanaman bergizi tinggi yang bermanfaat bagi kesehatan, namun keamanan dari infusa umbi bit masih belum diketahui. Penelitian ini bertujuan mengetahui toksisitas infusa umbi bit pada mencit mengunakan metode lethal dose 50 (LD50) dan mengetahui dampaknya terhadap fisiologis tubuh. Sebanyak 20 ekor mencit betina dewasa galur Deutschland Denken Yoken dibagi menjadi kelompok kontrol dan kelompok perlakuan yang diberi infusa umbi bit dengan dosis 5, 10, dan 15 g/kg BB secara oral. Parameter yang diamati yaitu gejala klinis, respons fisiologis, bobot badan, bobot organ absolut, bobot organ relatif, dan mortalitasnya selama 14 hari. Berdasarkan hasil penelitian didapatkan nilai LD50 dari infusa umbi bit yaitu 15,8 g/kg BB. Pengamatan terhadap respons fisiologis, bobot organ absolut, bobot organ relatif tidak menunjukkan perbedaan yang nyata (p > 0,05), namun pada kelompok pemberian dosis tinggi ditemukan perbedaan nyata terhadap bobot badan mencit tanpa menyebabkan gejala klinis dan perubahan makroanatomi organ tubuh. Infusa umbi bit termasuk ke dalam kategori praktis tidak toksik, tidak menimbulkan perubahan bobot organ, dan ditemukan perubahan gejala klinis seperti penurunan bobot badan mencit

Beetroot (Beta vulgaris) is a plant that high nutritional valueable for health, but the safety of beetroot infusion is still unknown. This study aims to determine the toxicity of beetroot infusion in mice using the lethal dose 50 (LD50) method and determine its impact on the physiological body. A total of 20 adult female mice of the Deutschland Denken Yoken strain were divided into control groups and and 4 groups treatment given beetroot infusion at doses of 5, 10, and 15 g/kg BW orally. Parameters observed were clinical symptoms, physiological responses, body weight, absolute organ weight, relative organ weight, and mortality for 14 days. Based on the results of the study, the LD50 of beetroot infusion was 15.8 g/kg BW. Observations of physiological responses, absolute organ weights, relative organ weights did not show significant differences (p>0.05), but in the high dose group there were significant differences in mice body weight without causing clinical symptoms and changes in organ macroanatomy. Beetroot infusion is included in the category of practically non-toxic, does not cause changes in organ weights, and changes in clinical symptoms such as a decrease in mice body weight are found.

Dr. drh. Andriyanto, M.Si