The Role of Vitekang Soil Conditioner and Arbuscular Mycorrhizae Fungi in Mitigating Cadmium Stress in <i>Solanum lycopersicum</i> Plants

<i>Solanum lycopersicum</i>, a widely cultivated vegetable crop globally, faces soil cadmium (Cd) contamination issues due to Cd’s high mobility, posing potential threats to <i>Solanum lycopersicum</i> growth and human health. In light of this, this study selected three representative <i>Solanum lycopersicum</i> varieties: Micro Tom, Red Guanyin, and Taiwan Pink King, and designed a series of experiments to investigate their growth performance under Cd stress. Experimental treatments included the sole application of different concentrations of Vitekang soil conditioner (VT), as well as the individual and combined application of VT and arbuscular mycorrhizal fungi (AMF). By thoroughly analyzing agronomic traits, cellular membrane lipid peroxidation levels, the activities of antioxidant enzymes (Catalase (CAT), Superoxide Dismutase (SOD), and Peroxidase (POD)), and the expression levels of genes related to Cd transport and detoxification (<i>SLNRAMP6</i> and <i>SlHMA3</i>), this study comprehensively evaluated the effectiveness of different treatments in mitigating Cd stress in the three <i>Solanum lycopersicum</i> varieties. The results indicated that when VT was applied at a concentration of 2.4 g/kg in combination with AMF, it significantly reduced the detrimental effects of Cd on Micro Tom, Red Guanyin, and Taiwan Pink King. The specific experimental outcomes were as follows: (i) significantly decreased Cd accumulation in <i>Solanum lycopersicum</i> roots and leaves; (ii) effectively mitigated cellular membrane lipid peroxidation; (iii) significantly increased antioxidant enzyme activities; and (iv) influenced expression patterns of genes related to Cd transport and detoxification. This study further confirms that, compared to the sole application of VT or AMF, the combined application of these two treatments serves as a more effective practical method, exhibiting significant advantages in alleviating soil Cd contamination, promoting <i>Solanum lycopersicum</i> growth, and improving agronomic traits. This study not only advances research progress on VT and AMF in <i>Solanum lycopersicumes</i>, providing a solid theoretical and experimental foundation for cultivating high-quality <i>Solanum lycopersicumes</i>, but also holds significant importance for improving and optimizing the “VIP+N” technology, achieving farmland soil protection, and enhancing agricultural product quality.