Comparative analysis of modified partial least squares regression and hybrid deep learning models for predicting protein content in Perilla (Perilla frutescens L.) seed meal using NIR spectroscopy
Kaur, Simardeep | Singh, Naseeb | Dagar, Preety | Kumar, Amit | Jaiswal, Sandeep | Singh, Binay K. | Bhardwaj, Rakesh | Rana, Jai Chand | Riar, Amritbir
Perilla seed meal (PSM), a byproduct of oil extraction from Perilla frutescens L. seeds, is rich in protein (24.26–42.85%) and holds potential as an economical and sustainable animal feed. Traditional methods for assessing protein content are labor-intensive and costly. This study explores Near-Infrared Reflectance Spectroscopy (NIRS) for the rapid, precise, and non-destructive determination of PSM protein content in 126 samples. We developed and evaluated Modified Partial Least Squares (MPLS) regression and deep learning (DL) models, including 1D-CNN (Convolutional Neural Network), LSTM Long Short-Term Memory), and hybrid architectures incorporating skip connections, inception modules, and spectral derivatives. Model performance was validated externally using parameters such as RSQexternal (R-squared), bias, SEP(C) (Standard Error of Prediction), RPD (Residual Prediction Deviation), slope, SD (Standard Deviation), p-value (≥0.05), and the correlation between reference and predicted values. The 1D CNN-LSTM-Inception derivative 1 model achieved the best performance (RPD: 8.0, RSQexternal: 0.98), followed by the MPLS-based model (RPD: 4.88, RSQexternal: 0.96) and the 1D CNN derivative 1 model (RPD: 3.07, RSQexternal: 0.96). These models provide a reliable and advanced technology for the non-destructive screening of PSM protein content, thus aiding in the rapid identification and selection of superior perilla chemotypes from varied backgrounds.
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