Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
2015
DINIZ, W. J. DA S. | TIZIOTO, P. C. | COUTINHO, L. L. | CESAR, A. S. M. | GROMBONI, C. F. | NOGUEIRA, A. R. DE A. | REGITANO, L. C. DE A. | WELLISON JARLES DA SILVA DINIZ, Federal University of São Carlos; POLYANA CRISTINE TIZIOTO; LUIZ LEHMANN COUTINHO, University of São Paulo; ALINE SILVA MELLO CESAR, University of São Paulo; CAIO FERNANDO GROMBONI, Bahia Federal Institute of Education; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Iron is essential to several biological processes, such as oxygen transport and lipid metabolism. In this study, the RNA sequencing technology (RNAseq) was adopted to identify differentially expressed genes (DE) and metabolic pathways, as well as its relationship with meat quality parameters influenced by the variation in iron content (IC) in muscle. Samples of Longissimus dorsi (LD) muscle from Nelore cattle (n = 8), extreme genomic estimated breeding value (GEBV) for IC (four samples with high (HIL) and four low (LIL) GEBV) were used. Data analysis by Tuxedo Suite identified 49 annotated genes DE between the groups. The functional enrichment analysis identified important metabolic pathways associated with lipid metabolism, like stearate biosynthesis, fatty acid biosynthesis and palmitate biosynthesis, which participate in the gene FASN, FABP4, SLC27A6 and THRSP. These results suggest biologic pathways influenced by iron and contribute information for the understanding of their participation in the process that affects muscle physiology in cattle.
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Эту запись предоставил Empresa Brasileira de Pesquisa Agropecuária