Development of transgenic wheat plants withstand salt stress via the MDAR1 gene
Mohamed Abdelsattar | Ahmed M. Ramadan | Amin E. Eltayeb | Osama M. Saleh | Fatthy M. Abdel-Tawab | Eman M. Fahmy | Sameh E. Hassanein | Hani M. Ali | Najla B. S. Al-Saud | Hussien. F. Alameldin | Sabah M. Hassan | Nermin G. Mohamed | Ahmed Z. Abdel Azeiz | Ahmed Bahieldin | Hala F. Eissa
In light of the fact that climate change has emerged as one of the difficulties confronting the global food system, researchers are obligated to work toward developing fundamental crops, particularly wheat, to combat environmental stress, including drought and salt. In the present study, genetic engineering was used to transfer the Arabidopsis MDAR1 gene, which controls the buildup of ascorbic acid (AsA) to make bread wheat less likely to be sensitive to salt stress. The biolistic bombardment was used to transfer cDNA from the Arabidopsis thaliana plant that encodes MDAR1 into Bobwhite 56 cultivar wheat plants. A molecular investigation was performed on six different transgenic lines to confirm the integration of the transgene, the copy number, and the expression of the transgene. There were one to three copies of the transgene, and there was no association found between the number of copies of the transgene and All the data generated or analyzed during this study are included in this published article [and its supplementary information files].the presence of its expression. Compared to plants that were not transgenic, the amount of ascorbic acid (AsA) that accumulated in the transgenic plants was twice as high. ROS concentrations are significantly lower in transgenic plants compared to non-transgenic plants under both control and salt stress conditions, effectively reducing oxidative stress. By cultivating transgenic T2 plants in a greenhouse, we were able to determine whether they were able to tolerate the potentially damaging effects of salt stress (200 mm). The study concluded that transgenic wheat plants that consistently expressed the MDAR1 gene become tolerant to salt stress with improvement in growth characteristics.
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