Piceatannol and other wine stilbenes: a pool of inhibitors against α-synuclein aggregation and cytotoxicity
Temsamani, Hamza | Krisa, Stéphanie | Decossas-Mendoza, Marion | Lambert, Olivier | Merillon, Jean-Michel | Richard, Tristan | Unité de Recherche Oenologie [Villenave d'Ornon] (OENO) ; Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV) | Institut des Sciences de la Vigne et du Vin (ISVV) | Chimie et Biologie des Membranes et des Nanoobjets (CBMN) ; Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011)
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Показать больше [+] Меньше [-]Английский. The aggregation of alpha-synuclein is one on the key pathogenic events in Parkinson's disease. In the present study, we investigated the inhibitory capacities of stilbenes against alpha-synuclein aggregation and toxicity. Thioflavin T fluorescence, transmission electronic microscopy, and SDS-PAGE analysis were performed to investigate the inhibitory effects of three stilbenes against alpha-synuclein aggregation: piceatannol, ampelopsin A, and isohopeaphenol. Lipid vesicle permeabilization assays were performed to screen stilbenes for protection against membrane damage induced by aggregated alpha-synuclein. The viability of PC12 cells was examined using an MTT assay to assess the preventive effects of stilbenes against alpha-synuclein-induced toxicity. Piceatannol inhibited the formation of alpha synuclein fibrils and was able to destabilize preformed filaments. It seems to induce the formation of small soluble complexes protecting membranes against alpha-synuclein-induced damage. Finally, piceatannol protected cells against alpha-synuclein-induced toxicity. The oligomers tested (ampelopsin A and hopeaphenol) were less active.
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