ISOLATION AND IDENTIFICATION OF SALMONELLA SPP. FROM POULTRY FARMS BY USING DIFFERENT TECHNIQUES AND EVALUATION OF THEIR ANTIMICROBIAL SUSCEPTIBILITIES
2014
Ali A. AL-Iedani | Nael M. Oufi | Mohammed H. Khudor
This study aimed to isolate and identify Salmonella spp. from various sources of poultry farms by using four different techniques (conventional biochemical tests, API 20E system, serology and polymerase chain reaction) the total number of isolates was 44(9%). The Salmonellae including 4 species, S. gallinarum 9(1.85%), S. typhimurium 7(1.44%), S. newport 21(4.3%) and S. ohio 1(0.21%). The highest isolation rate was in first week of chicks life 18(25.7%), however, the highest isolation rate of salmonella was from liver 13(28.8%). There are similarity in identification rate of Salmonella spp. between API 20 E system and PCR assay using flic gene. In this study using PCR amplification of rfbsg and rfbsp genes in differentiation of Salmonella serovar gallinarum into S. gallinarum and S. pullorum biovars very useful. Results of antimicrobial susceptibility revealed high resistance of isolates against seven antibiotics arranged in descending from high to low resistance (Azithromycin, Florfenicol, Trimethoprime-sulphamethaxezole, Tetracycline, Ciprofloxacin, Ampicillin and Gentamycin).
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