ANTIBACTERIAL AND ANTI INFLAMMATION ACTIVITY OF Salix EXTRACTION on staphylococcus aureus ISOLATED FROM DIFFERENT CUTANEOUS INFECTION
2016
Rana Faaz
This study was conducted to elevated the ethanol and methanol of Salixa acmophyllaextraction on bacteria Staphylococcus aureus isolated from skin wound of cattle, about100(66.66%)from 150 sample isolated , and made the biochemical test , also sensitivity ofantibiotic .The loaded antibiotic discs namely: gentamicin (CN-10 µg), levofloxacin(LEV -5µg), amoxicillin (Ax -25 µg) , tetracycline (TE -30 µg),vancomycin(30µg),erythromycin (25µg)and streptomycin(25 µg) were placed on thesurface of the medium and left for 30 min at room temperature for complete diffusion. Theplates were incubated for 24 hrs. at 37°C. also made antimicrobial against isolatedbacteria disk diffusion techniques , Minimum inhibition concentration (MIC) with differentconcentration of plant extracts (25mg /ml ,50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg /mland 300, . Minimum bacterial concentration (MBC) by Macro-dilution broth method weredone . Anti-inflammation test albumin denaturation Maximum inhibition in case ofethanol extract about (65.25±1.2 %) and in methanol extract about (58.135±1.23) wasobserved at concentration 300 µg/ml., Aspirin, a standard anti-inflammatory drug showedthe maximum inhibition ، (67.35±0.56%) at the concentration of (100µg/ml )
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