EVALUATION OF INTRAYOLK SAC INOCULATION OF INFECTIOUS BURSAL DISEASE VACCINE ON IMMUNE RESPONSES IN NEWLY HATCHED BROILER CHICKS

Harith A. Najem

EVALUATION OF INTRAYOLK SAC INOCULATION OF INFECTIOUS BURSAL DISEASE VACCINE ON IMMUNE RESPONSES IN NEWLY HATCHED BROILER CHICKS

2018

Harith A. Najem

A total of 60 one-day old Ross broiler chicks were used in this study. The birds were divided into four groups. The first group A was vaccinated against IBD with a Cevac IBD L vaccine at one-day old by intra-yolk sac method (lYS) with a dose of 0.5ml using a (1ml) syringe, whereas the second group B was vaccinated against IBD with the same vaccine at 14 days of age by drinking water method (DW). The third group C and fourth group D were considered as a positive and negative control respectively . On day 35, the birds were weight and killed to collect blood samples and lymphoid organs were removed and weight to study the effects of (lYS) rout on body weight , lymphoid organ weights and the humeral immune response to IBD vaccine which measured by ELISA test. The results showed significant differences at (P≤0.05) in body weight of group A, in addition significant (p<0.05) increase in the all lymphoid organs weight , Bursa of fabricius (BF) was 1.643 a ±0.066 in group comparison with other groups which had been recorded 1.102 b ±0.022, 0.850 c ±0.067 in group B and C respectively. Group B indicated that was a significant decrease at (p<0.05) in the weight of of spleen which was 1.102 b ± 0.022 in comparison to control groups. With regard to serological test, bird from group A recorded higher Ab titer 24038 a± 685 compared to those of other groups , and this increment was statically significant (p<0.05), however both group A and B revealed a53 significant differences( p <0.05) from the control groups. Generally the results indicated that intra-yolk sac method was highly effective rout of IBD vaccine administration

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