DIFFERENTIAL EXPRESSION OF SECRETED ASPARTYL PROTEASE (SAP8 AND SAP10) GENES AND COMPARATIVE PATHOGENICITY OF Candida albicans GROWN IN VARIOUS ENVIRONMENTAL CONDITIONS
2020
Inas K. Rahem | Hayder M. Samaka | Hutheyfa A. Al Salih
This study focusing on the importance of Candida albicans growth conditions on SAP8and SAP10 genes expression, as a member of the Secreted Aspartic Proteases superfamily genesthat play a role in the pathogenicity of C. albicans and the effects of this conditions on thepathogenicity of this bacterium in albino mice.Ten pathogenic isolates of C. albicans weregrown on two different conditions using RPMI1640 medium at 37° C for mimic host conditionand on Sabouraud Dextrose Agar (SDA) at 25° C as in vitro condition. Sets of primers were usedto detect SAP8 and SAP10 genes expression in each condition. Forty BALB/c albino mice wereassigned to groups and inoculated with 1 X 107 cells/mouse of C. albicans grown in the studyconditions as challenge dose. Kidneys, lungs, and liver were collected to study the pathologicalchanges. Data show overexpression of the SAP8 gene in study isolates grown in RPMI1640comparison to the SAP10 gene. Kidneys, liver, and lungs showed pathological lesions at adifferent range of severity, a significant severe lesion in the kidney in mice injected with C.albicans grown in RPMI 1640 medium, while in contrast the significant severe liver and lunglesions were observed in mice injected with C. albicans grown in SDA medium. This studypoints out that the growth condition of C. albicans plays a role in the pathogenicity of thismicroorganism and SAP8 gene related to the infection process in the host.
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