Molecular Detection of Fasciola gigantica Using a Conventional PCR Assay in Basrah Province
2024
Fatima Zahra Aqeel Hassan | Suzan Al-Azizz
Fascioliasis represents an ignored zoonotic disease that results from liver flukes that belong to the genus Fasciola spp. It results in substantial financial damages in the cattle sector.In the current investigation, liver flukes were taken from cattle in two places: the Basrah abattoir and the Aljazeera region. The flukes were found on a buffalo and a cow. The nuclear marker genes were used to identify the species and learn about the past of Fasciola spp. spread.From a total number of examined Buffalo (11) and Cow ( 11), the infected with Fasciola spp. were in Buffalo (2) and in Cow (9), and the percentage of infection was 18% and 81%, respectively.The PCR results showed that all examined samples by PCR were found as gigantic Fasciola, with an identity of 100% according to GenBank. In our current study, we isolated Fasciola spp. Two worms from sheep and PCR were done, and it was observed that it is F. gigantica. This indicates that the distribution of the parasite is emerging from the host specificity, which means it must be molecularly done in detail studies in all types of cattle, not only in Basrah city but all over Iraq.
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