Staphylococcus aureus RNAIII coordinately represses the synthesis of virulence factors and the transcription regulator Rot by an antisense mechanism.
2007
Boisset, Sandrine | Geissmann, Thomas | Huntzinger, Eric | Fechter, Pierre | Bendridi, Nadia | Possedko, Maria | Chevalier, Clément | Helfer, Anne Catherine | Benito, Yvonne | Jacquier, Alain | Gaspin, Christine | Vandenesch, François | Romby, Pascale | Immunité infection vaccination (I2V) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM) | Architecture et réactivité de l'ARN (ARN) ; Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) | Génétique des Interactions Macromoléculaires ; Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) | Unité de gestion du département de biométrie et intelligence artificielle ; Institut National de la Recherche Agronomique (INRA)
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Показать больше [+] Меньше [-]Английский. RNAIII is the intracellular effector of the quorum-sensing system in Staphylococcus aureus. It is one of the largest regulatory RNAs (514 nucleotides long) that are known to control the expression of a large number of virulence genes. Here, we show that the 3' domain of RNAIII coordinately represses at the post-transcriptional level, the expression of mRNAs that encode a class of virulence factors that act early in the infection process. We demonstrate that the 3' domain acts primarily as an antisense RNA and rapidly anneals to these mRNAs, forming long RNA duplexes. The interaction between RNAIII and the mRNAs results in repression of translation initiation and triggers endoribonuclease III hydrolysis. These processes are followed by rapid depletion of the mRNA pool. In addition, we show that RNAIII and its 3' domain mediate translational repression of rot mRNA through a limited number of base pairings involving two loop-loop interactions. Since Rot is a transcriptional regulatory protein, we proposed that RNAIII indirectly acts on many downstream genes, resulting in the activation of the synthesis of several exoproteins. These data emphasize the multitude of regulatory steps affected by RNAIII and its 3' domain in establishing a network of S. aureus virulence factors.
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