Isolation of Microsatellite DNA and Preliminary Genomic Analysis of Mud Carp ( Cirrhina molitorella )
2007
CHENG, Fei | YE, Wei | YE, Fu-liang
In order to determine the applicability of microsatellite primers developed from common carp ( Cyprinuscario ) for genomic analysis in mud carp ( Cirrhina olitorella ), 24 primer pairs from common carp were designed to amplify microsatellite loci in the mud carp containing CA, GA, AT and GGGA sequences. Thirteen primers (54%) successfully amplified specific products in the mud carp and 11 primers (48%) showed high polymorphism in the mud carp population. The results indicated that the average number of alleles per locus in the mud carp stocks was 5.2. Average heterozygosity (Ho), unbiased expected heterozygosity (Ho) and polymorphism information content (PIC) in the wild population were 0.61±0.2, 0.8±0.09 and 0.72±0.1 respectively. Several Hardy-Weinberg departure value were significantly departed from Hardy-Weinberg equilibrium. The study showed that microsatellite primers from a species of Cyprinidae can be used for mud carp genetic analysis without much cost or time input.
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