Optimizing CuSO4 and CoCl2 for Superior Somatic Embryos Regeneration of Date Palm
Sara Abdelmotagaly | Yasmin Abdellatif | Amal Zein Eldin | Ola Abd Elbar | Ibrahim Ibrahim
The purpose of the research was to improve the morpho-anatomical and physiological features of somatic embryos (SEs) of date palms in tissue culture media that have the potential to produce green and healthy plantlets. Copper sulfate (CuSO4) and cobalt chloride (CoCl2) were supplemented to date palm maturation medium at various concentrations, separately or combined. Adding CoCl2 to the media of maturation produced the greatest relative growth and differentiation percentages of date palm embryogenic calli, particularly at 0.52 mgL-1. In comparison to the control, when combined treatments were administered alone, the quantity of the produced somatic embryos rose dramatically (17/jar). Total sugars and free amino acids were the highest in SEs grown on a maturation medium with CoCl2 at 0.26 mgL-1. Moreover, total phenols and indoles were picked significantly at their higher levels in the generated SEs that were cultured on a medium enhanced with 0.52 mgL-1. When the maturation medium was administered along with both as a treatment, the antioxidant enzyme, ascorbate peroxidase, reached its maximum activity. Whereas, adding 0.52 mgL-1 of CoCl2 to the culture medium enhanced the activities up to of highest levels of ascorbate peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase.
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