CbCBF2 Integrates JA and BR Signaling to Enhance Oleanolic Acid Biosynthesis in <i>Conyza blinii</i> H. Lév Under Cold Stress
Ming Yang | Guodong Zhang | Junjie Deng | Tianrun Zheng | Moyang Liu
Low temperature significantly contributes to the medicinal quality of <i>Conyza blinii</i>. The CBF/DREB1-dependent cold-responsive signaling pathway is a major contributor to plant cold stress resistance. However, whether the CBF/DREB1 signaling pathway affects the terpenoid metabolism of <i>C. blinii</i> under cold stress remains to be explored. Here, we systematically identified and analyzed the impact of CbCBFs on the terpenoid metabolism of <i>C. blinii</i>. The results showed that three <i>CbCBFs</i> and <i>CbICE1</i> were identified based on the transcriptome. The functions significantly correlated with <i>CbCBFs</i> encompass plant hormones and stress responses. Co-expression analysis revealed that key genes in BR and JA signaling pathways were correlated with <i>CbCBFs</i>. Among them, CbCBF2 is the predominant factor under low-temperature conditions and is significantly positively correlated with oleanolic acid. Overexpression of <i>CbCBF2</i> significantly upregulated the catalase gene <i>CbβAS</i> and increased oleanolic acid content in the leaves. These results indicate that CbCBF2 can act as a major regulatory factor to promote the synthesis of oleanolic acid by integrating JA and BR signaling under low temperature conditions.
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