Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch
Mingmei Wu | Rui Dong | Yan Zhang | Haojie Liao | Tian Tian | Dandan Xu | Youjun Zhang | Zhaojiang Guo | Shaoli Wang
Pesticide resistance greatly limits control efficacy after the long-term application of pesticides. The two-spotted spider mite, Tetranychus urticae Koch, is a notorious agricultural pest worldwide that is resistant to various pesticides, including abamectin. While some studies of abamectin resistance have investigated target resistance related to glutamate-gated chloride channels (GluCls), studies on the metabolic resistance mechanisms are still limited. In this study, we identified an ABCC subfamily gene, TuABCC4, that was overexpressed in resistant populations of T. urticae, based on the analysis of previously obtained transcriptomic and RNA-seq data. No consistent nonsynonymous mutations in the TuABCC4 gene were found between the susceptible and resistant populations, although TuABCC4 expression was significantly increased in all the resistant populations that were studied. Synergistic experiments with the inhibitor verapamil and gene expression analysis of the susceptible and resistant populations confirmed the key role of TuABCC4 in abamectin resistance. In addition, an increase in the expression of the TuABCC4 gene was shown by RNA interference and genetic association analysis to be closely related to the resistance of T. urticae to abamectin. In conclusion, overexpression of TuABCC4 was shown to be involved in abamectin resistance in T. urticae. These results can help us to better understand the molecular basis of pest resistance to abamectin.
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