EFFECT OF 17β-ESTRADIOL HORMONE ADMINISTRATION ON THE DOG CONCH’S Laevistrombus turturella GONAD DEVELOPMENT | PENGARUH PEMBERIAN HORMON 17β-ESTRADIOL TERHADAP PERKEMBANGAN GONAD SIPUT GONGGONG Laevistrombus turturella

Английский. Dog conch Laevistrombus turturella is captured intensively, therefore, their population is decline. The dog conch culture is needed to maintain their population. The technology towards conch reproduction is still not developed yet in Indonesia. The 17β-estradiol hormone in conch and some mollusks has been stated by some researcher yet its main role in conch reproduction process has not widely known. The study about the addition of 17β-estradiol hormone in accelerating gonad development has never been reported. The aim of this study was to evaluate the impact of the addition of 17β-estradiol against dog conch’s gonad development. This study used three treatments for three groups of dog conch those were without injection (P1), injection by 30 µL of corn oil mixed with absolute ethanol (P2), and 30 µL of 17β-estradiol stock solution (P3). After injection, the dog conch was reared in pens culture in their natural habitat for 30 days. This study showed that the injection of a 17β-estradiol solution (P3) stimulated the dog conch’s gonad development as evidenced by greater oocyte mean diameter than another treatment. The mean of gonadal weight and GSI on P3 treatment was also higher than treatments P1 and P2. SDS-PAGE analysis showed that dog conch’s hemolymph has several kinds of proteins with varying molecular weights. Proteins with a molecular weight of 54-55 kDa are predicted as dog conch’s vitellogenin.

индонезийский. Siput gonggong Laevistrombus turturella semakin intensif ditangkap sehingga populasinya di alam menurun. Upaya budidaya gonggong diperlukan untuk menjaga populasinya. Teknologi reproduksi siput belum banyak dikembangkan di Indonesia.  Keberadaan 17β-estradiol pada siput dan moluska lain telah dinyatakan oleh beberapa peneliti, namun perannya dalam proses reproduksi siput belum banyak diketahui. Penelitian tentang pemberian 17β-estradiol untuk memacu perkembangan gonad gonggong belum pernah dilaporkan. Penelitian ini bertujuan mengaji pengaruh pemberian 17β-estradiol terhadap perkembangan gonad gonggong. Ada tiga perlakuan yang diberikan pada tiga kelompok gonggong, yaitu masing-masing tanpa suntikan (P1), suntikan 30 µL/ekor larutan minyak jagung dan etanol absolut (P2), serta suntikan 30 µL/ekor larutan 17β-estradiol (P3). Pascasuntikan, gonggong dipelihara dalam pensculture di habitat alaminya selama 30 hari. Hasil penelitian menunjukkan bahwa perlakuan suntikan larutan 17β-estradiol (P3) menstimulasi perkembangan gonad gonggong yang dibuktikan dengan nilai rata-rata ukuran diameter oosit yang lebih besar daripada perlakuan lain. Nilai rata-rata bobot gonad dan GSI perlakuan P3 juga lebih besar dibanding perlakuan P1 dan P2. Analisis SDS-PAGE menunjukkan hemolimfa gonggong memiliki beberapa jenis protein dengan berat molekul bervariasi. Protein dengan berat molekul 54-55 kDa diprediksi sebagai vitelogenin gonggong.