ISOLATION, IDENTIFICATION AND MOLECULAR DETECTION OF E. coli FROM AN OUTBREAK OF COLIGRANULOMA IN WHITE LEGHORN LAYER CHICKEN
2022
J, Venkatesh Yadav | G, Sairam Sandeep | T. R., Kannaki
Coligranuloma is an economically important bacterial disease caused by Escherichia coli and is characterized by nodular granulomas in liver, mesentery and walls of intestine. Three adult white leghorn layer birds of 24-week-old were brought for necropsy examination from Jeelakarragudem village of West Godavari district. Upon necropsy, the birds revealed typically hard, yellow, nodular granulomas in the mesentery and on the wall of the intestines with few tiny nodular lesions on the liver. Impression smears of the nodules showed large number of gram-negative rod-shaped organisms. The inoculums from the nodular lesions when streaked on to MacConkey agar and EMB agar resulted in characteristic rose-pink and metallic sheen colonies respectively. PCR targeting the E. coli 16S ribosomal DNA resulted in an amplicon of 585 bp. Based on the necropsy lesions, staining and cultural characteristics and PCR, the disease was confirmed as Coligranuloma.
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