Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates
Gimeno, Mariona | Darwich, Laila | Diaz, Ivan | de la Torre, Eugenia | Pujols, Joan | Martín, Marga | Inumaru, Shigeki | Cano, Esmeralda | Domingo, Mariano | Montoya, Maria | Mateu, Enric | Producció Animal | Sanitat Animal
The present study examined the immunological response of antigen presenting cells (APC) to genotype-I isolates of porcine reproductive and respiratory syndrome virus (PRRSV) infection by analysing the cytokine profile induced and evaluating the changes taking place upon infection on immunologically relevant cell markers (MHCI, MHCII, CD80/86, CD14, CD16, CD163, CD172a, SWC9). Several types of APC were infected with 39 PRRSV isolates. The results show that different isolates were able to induce different patterns of IL-10 and TNF-a. The four possible phenotypes based on the ability to induce IL-10 and/or TNF-α were observed, although different cell types seemed to have different capabilities. In addition, isolates inducing different cytokine-release profiles on APC could induce different expression of cell markers.
Показать больше [+] Меньше [-]We are grateful to Laura Martínez for her assistance in detection of TTV, Núria Navarro for her technical assistance. Antibodies for SLA-I, SLA-II, CD163 and SwC3 were kindly provided by Dr J. Dominguez (INIA, Madrid). This study has been funded by projects AGL2005-07073-C02-02, AGL2006-13809- C02-01 and AGL2008-05708-C02-02 of the Spanish Ministry for Science and Innovation (MCINN). Mariona Gimeno received a grant from the Universtitat Autonoma de Barcelona (PIF). Ivan Diaz was funded by project ConsoliderIngenio 2010 CDS2006-00007-Porcivir (MCINN).
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