ISOLATION AND IDENTIFICATION OF LOCAL PGPR ISOLATES AND EVALUATION OF THEIR EFFICIENCY IN INHIBITING THE PATHOGENIC FUNGUS RHIZOCTONIA SOLANI ON BROAD BEAN IN LABORATORY CONDITIONS
S. H. A. AL-Mayahi | A. K. Hassan | S. A. H. Alamery
This study was conducted to evaluate the efficiency of some PGPR isolates as biological agents in controling root rot disease (Rhizoctonia in broad beans) caused by the fungus Rhizoctonia solani. Tweenty six isolates were obtained through the Plant Growth Promoting Rhizobacteria sampling from different areas. Two isolates demonstrated a high antagonistic activity against the pathogenic fungus isolate. The identification of two isolates were Alcaligenes faecalis and Acinetobacter radioresistens was confirmed using vitek technology and nucleotide sequencing. The two strain sequences were deposited in the Gen Bank under accession numbers AR1 (OR101916) and AR2 (OR101917). The antagonistic activity was 100% for both isolates in the first method according to (Al-Mayahi and Hassan, 2021). While in the second method according to (Raspor at all, 2010), the two isolates of A. faecalis and A. radioresistens scored 58.92 and 57.14% inhibition at concentration 10-7 and 10-6, respectively. Secondary metabolites test of A. faecalis and A. radioresistens scored 93.33 and 85.55% inhibition rate at a concentration 10%
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