Characterizing morphological heterogeneity in porcine hepatocytes
KIRPAL SINGH | VARINDER UPPAL | NEELAM BANSAL | ANURADHA GUPTA | DEVENDRA PATHAK
The present study was conducted on liver of eight pigs collected from local slaughter house, for gross anatomical, histological, histochemical, immunohistochemical and histoenzymic studies. The paraffin sections of 3 µm were cut and stained with different histomorphochemical stains. The cryostat sections of fresh frozen tissues of 10 µm thickness were cut at -20˚C and incubated in different substrates to localize phosphatases and Non-specific esterases. The left lateral lobe was significantly larger and quadrate lobe was shortest. The size of hepatocytes in periportal area was significantly more than size of hepatocytes in centrilobular area. The number of mononuclear hepatocytes was significantly more in periportal area than centrilobular area whereas number of binucleated hepatocytes didn’t vary significantly between periportal and centrilobular areas. The average no. of Kupffer cells in periportal region was significantly more than centrilobular area. The reaction for neutral mucopolysaccharides and glycogen was more in centrilobular hepatocytes, proteins were distributed homogeneously and reaction for lipids was more in centrilobular hepatocytes. Periportal hepatocytes exhibited a strong phosphatases activity whereas non-specific esterases activity was more in centrilobular hepatocytes. This work will contribute to the corpus of knowledge regarding the heterogeneity of hepatocytes, which enables the liver to carry out a variety of functions and enhances its complexity and adaptability in preserving the body’s homeostasis.
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